Lab mishaps
It's been almost two full years since i started work in Dr Chew's lab. Today is my "unofficial" official last day. Would have been so on wednesday if not for a set of controls i forgot to include. (what's new?) Even like after so long, i'm still rebuked by L for some things i ought to have done properly.
"Xiuying, next time please remember to close the -80 door latch properly."
"Oh, you mean the door inside is it? ok.. ok.." (smiles cheezily and resumes filling some container with distilled water like oh-it's-really-so-difficult-i-need-to-focus)
Ai yo, after so long, just when i thought "yes i've grown!", someone pours cold water and reminds me about these nitty gritty details. Talk about having humility!
Which brings me down memory lane to recall some of those instances that has forever condemned me to be a klutz at work, plus guaranteed chop!
1. plasmid extraction, pouring two different plasmids into the same eppendorf. Oops! There goes one week of effort.
2. leaving the extracted plasmid on benchtop overnight and worrying through the night "Oh no, is my DNA just going to disintergrate?"
3a. using PVDF membranes without activating in methanol over two/three separate occasions till JN comes along and asks me "you mean you don't dip into methanol before using?". Oh great! i spent two weeks trying to figure why proteins just won't transfer.
3b. Forgetting to mark on the membrane and when X-rayed - Oops, which protein is which band?
4. leaving bacteria to grow over one entire week in incubator till JS comes and asks if i still want it. Heh heh, actually need to throw...
5. letting the cleaned up DNA dry at laminar hood overnight but still send for sequencing anyway. Phew, it still works, thank God.
6. Seeing the protein sample float up when loading into gel. Argh!!! there goes my sample. Actually, it's the buffer's fault. Too concentrated.
7. Always scolded for scanning pictures.
8. using open 96-well plates for sterile work. Boy! How stupid can i get?
9. Expecting the LB agar to liquefy entirely after 10 min in microwave oven and upon opening the door... Argh!!!! the agar burnt! U have to monitor the liquid every 2-3 min!
10. Spending half a year just to get the best protocol to express proteins
Hallelujah Praise the lord! Thank God i'm still alive.
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